Abstract
The Brazilian regions are still highly endemic areas for Canine morbillivirus [canine distemper virus (CDV)]. However, little is known regarding the genetic variability of the strain circulating in several Brazilian regions. Here, we report the first full-length genome and molecular characterization of CDV isolated from domestic dogs in the Brazilian Center-West region. Sequence alignment and phylogenetic analyses based on deduced amino acid and nucleotide sequences showed that the isolated strain is characterized as the South America-I/Europe genotype. However, it segregates into a CDV subgenotype branch. Interestingly, both H and F proteins have a gain of a potential N-glycosylation sites compared to the Onderstepoort vaccine strain. Therefore, this study provides a reference to further understand the epidemic and molecular characteristics of the CDV in Brazil.
Highlights
The Brazilian regions are still highly endemic areas for Canine morbillivirus [canine distemper virus (CDV)]
CDV infection can result in canine distemper (CD), with clinical signs including the onset of cutaneous rash, serious nasal and ocular discharge, conjunctivitis, and anorexia, followed by gastrointestinal and respiratory signs[1,4,5]
While Brazil is considered endemic for CD, with high disease incidence rates[15], there have been limited studies conducted in the country related to virus isolation and molecular characterization of the circulating wild-type strains
Summary
The Brazilian regions are still highly endemic areas for Canine morbillivirus [canine distemper virus (CDV)]. We report the first full-length genome and molecular characterization of CDV isolated from domestic dogs in the Brazilian Center-West region. Sequence alignment and phylogenetic analyses based on deduced amino acid and nucleotide sequences showed that the isolated strain is characterized as the South America-I/Europe genotype. It segregates into a CDV subgenotype branch. While Brazil is considered endemic for CD, with high disease incidence rates[15], there have been limited studies conducted in the country related to virus isolation and molecular characterization of the circulating wild-type strains. To elucidate the genetic basis of the protein diversity of CDV, we conducted amino acid and nucleotide sequence analysis of a recent field isolate, with a focus on the H and F genes, which are the most suitable targets to investigate the CDV variability and evolution[5,13,14]
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