Abstract
BackgroundAgro-industrial waste from tropical environments could be an important source of lactic acid bacteria (LAB) with probiotic potential.MethodsTwelve LAB isolates were isolated from pineapple silages. The species identification was carried out considering 16S rRNA and pheS genes. Experiments to evaluate the probiotic potential of the isolates included survival under simulated gastrointestinal environment, in vitro antagonistic activity (against Salmonella spp. and Listeria monocytogenes), auto-aggregation assays, antibiotic susceptibility, presence of plasmids, adhesiveness to epithelial cells, and antagonistic activity against Salmonella in HeLa cells.ResultsLacticaseibacillus paracasei, Lentilactobacillus parafarraginis, Limosilactobacillus fermentum, and Weissella ghanensis were identified. Survival of one of the isolates was 90% or higher after exposure to acidic conditions (pH: 2), six isolates showed at least 61% survival after exposure to bile salts. The three most promising isolates, based on survivability tests, showed a strong antagonistic effect against Salmonella. However, only L. paracasei_6714 showed a strong Listeria inhibition pattern; this isolate showed a good auto-aggregation ability, was resistant to some of the tested antibiotics but was not found to harbor plasmids; it also showed a high capacity for adhesion to epithelial cells and prevented the invasion of Salmonella in HeLa cells. After further in vivo evaluations, L. paracasei_6714 may be considered a probiotic candidate for food industry applications and may have promising performance in acidic products due to its origin.
Highlights
The development and intake of functional foods containing probiotic microorganisms have grown considerably due to their known health benefits and ability to prevent certain diseases (Nami et al, 2018)
A total of twelve different lactic acid bacteria (LAB) morphotypes were isolated from twenty pineapple silages with increasing levels of urea
Considering the 16S rRNA sequence and phenylalanine-tRNA ligase subunit alpha (pheS) gen the isolates correspond to L. paracasei, Lentilactobacillus parafarraginis, Limosilactobacillus fermentum, and W. ghanensis (Table 1 and Table S1)
Summary
The development and intake of functional foods containing probiotic microorganisms have grown considerably due to their known health benefits and ability to prevent certain diseases (Nami et al, 2018). Experiments to evaluate the probiotic potential of the isolates included survival under simulated gastrointestinal environment, in vitro antagonistic activity (against Salmonella spp. and Listeria monocytogenes), auto-aggregation assays, antibiotic susceptibility, presence of plasmids, adhesiveness to epithelial cells, and antagonistic activity against Salmonella in HeLa cells. Paracasei_6714 showed a strong Listeria inhibition pattern; this isolate showed a good auto-aggregation ability, was resistant to some of the tested antibiotics but was not found to harbor plasmids; it showed a high capacity for adhesion to epithelial cells and prevented the invasion of Salmonella in HeLa cells. Paracasei_6714 may be considered a probiotic candidate for food industry applications and may have promising performance in acidic products due to its origin After further in vivo evaluations, L. paracasei_6714 may be considered a probiotic candidate for food industry applications and may have promising performance in acidic products due to its origin
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