Abstract
This report describes the first autochthonous case of canine visceral leishmaniasis in Rondônia, northern Brazil. A canine resident of the municipality of Cacoal, with clinical signs and symptoms of visceral leishmaniasis, was treated by a veterinarian. Samples were analyzed by a reference laboratory. Dual-path platform (DPP) assay, indirect immunofluorescence technique (IIT), enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), isolation in a culture medium, and direct parasitological analysis were performed. DPP assay, IIT, and ELISA revealed positive results for Leishmania; PCR identified the species as Leishmania infantum. Based on the clinical presentation and test results, canine visceral leishmaniasis was diagnosed.
Highlights
Canine visceral leishmaniasis (CVL) is a worldwide public health concern, and areas at risk for the incidence of this disease are expanding[1]
A blood sample was collected from the animal by a veterinarian and sent to a laboratory accredited by the Minas Gerais Canine Visceral Leishmaniasis Diagnostic Laboratory Network; accreditation was performed as recommended by the Ministry of Health [Ministério da Saúde (MS)]
Blood samples collected from the other canines were tested using Dual-path platform (DPP) assay and enzymelinked immunosorbent assay (ELISA), and one of the dogs tested positive in the DPP assay. This is the first report of an autochthonous case of CVL in the Rondônia State, and the case identification was based on the clinical, laboratory, and epidemiological findings
Summary
Canine visceral leishmaniasis (CVL) is a worldwide public health concern, and areas at risk for the incidence of this disease are expanding[1]. Abstract This report describes the first autochthonous case of canine visceral leishmaniasis in Rondônia, northern Brazil. A canine resident of the municipality of Cacoal, with clinical signs and symptoms of visceral leishmaniasis, was treated by a veterinarian.
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