Firing rate trajectories of human motor units during isometric ramp contractions to 10, 25 and 50% of maximal voluntary contraction

Abstract

During low torque graded isometric contractions, motor units (MU) exhibit initial firing rate acceleration followed by saturation demonstrating a non-linear response attributed to persistent inward currents (PICs) which contribute to the net excitatory input. Firing rate saturation studies have been done exclusively at recruitment thresholds of low firing threshold MUs below 10% of isometric maximal voluntary contraction(MVC). It remains unclear whether later recruited (i.e. higher-threshold) MUs follow a similar firing rate trajectory as low-threshold units. Thus, MU firing rate trajectories were explored in relation to MU recruitment threshold (RT) at contraction levels between 10 and 50% of MVC. During graded isometric contractions to 10, 25 and 50% of MVC, single MU potentials were recorded from the tibialis anterior from 5 participants using tungsten microelectrodes. To characterize the firing rate trajectory, each MU train was fit by competing functions of torque as an exponential (i.e. saturated) and simple linear regression, using previous analysis methods (Fuglevand et al. 2015). Throughout a RT range of 0.02–41% of MVC, 261 MUs were compared. In 87% of MUs the better fit was by a linear function, whereas the remaining MUs (13%) were fit better with an exponential (saturated) firing rate trajectory. There was no statistical difference in the number of MUs better fit by the exponential function between low (<10% MVC) and relatively higher threshold MUs (>10% MVC; both p < 0.05). Increasing RT and rate of torque development (RTD) of the ramps were correlated with increased firing rate variability (larger error) in both fits (r = 0.3 and r = 0.4, both p < 0.01). Additionally, there was a 4-fold increase in peak antagonist surface electromyography (EMG) from 10 to 50% MVC contraction ramps. When all MUs were plotted with a normalized firing onset (i.e. 0% MVC) the data visually displayed an initial firing rate acceleration followed by a linear response (biphasic trajectory). Increased synaptic drive and greater antagonist surface EMG during moderate torque outputs may dampen PIC activity as compared with MUs during lower torque (<10% MVC) recruitment levels.