FINITE ELEMENT ANALYSIS OF MECHANICAL DEFORMATION OF CHONDROCYTE TO 2D SUBSTRATE AND 3D SCAFFOLD

Abstract

The mechanical properties of cells are important in regulation of many aspects of cell functions. The cell may respond differently to a 2D plate and a 3D scaffold. In this study, the finite element analysis (FEA) was adopted to investigate mechanical deformation of chondrocyte on a 2D glass plate and chondrocyte seeded in a 3D scaffold. The elastic properties of the cell differ in these two different compression tests. This is because that the cell sensed different environment (2D plate and 3D construct) which can alter its structure and mechanical properties. It reveals how the apparent Poisson's ratio of a cell changes with the applied strain depends on its mechanical environment (e.g., the elastic moduli and Poisson's ratios of the scaffold and extracellular matrix) which regulates cell mechanics. In addition, the elastic modulus of the nucleus also plays a significant role in the determination of the Poisson's ratio of the cell for the cells seeded scaffold. It also reveals the intrinsic Poisson's ratio of the cell cannot be obtained by extrapolating the measured apparent Poisson's ratio to zero strain, particularly when scaffold's Poisson's ratio is quite different from the cell.