Abstract
Recently, many aspects of FecB gene, including reproductive endocrinology, organs development and body mass have been studied. FecB has an additive effect on litter size and ovulation. The present investigation was carried out to study polymorphism by forced PCR-RFLP of FecB gene in five Egyptian local sheep breeds and its comparison with other foreign sheep breeds. Genomic DNA was isolated from a total of 100 animals of Egyptian sheep breeds namely Rahmani, Ossimi, Awassi, Barki and Awassi x Barki crossbred. Forced PCR of the FecB gene 190 base pair (bp) was amplified using specific primer designed to introduc a point mutation in the resulting PCR products with FecB carrier sheep containing an AvaII restriction site (G|GACC), whereas products from noncarriers lacked (of) this site. Digestion of FecB gene 190 base pair with AvaII restriction enzyme resulted in non carrier 190 bp band (wild type) in all the animals belonging to the five Egyptian breeds studied revealing absence of this restriction site in those five breeds. .
Highlights
Sheep contribute 6% of the total red meet produced in Egypt
Ossimi, and Barki, are of the main sheep breeds in Egypt with a population of 990,000, 514,000, and 470,000 respectively (Galal et al, 2005).Regarding the economic importance of sheep in meat production in Egypt, it becomes essential to make fingerprinting of some genes related to economic traits such as litter size and growth rate in order to determine the polymorphism pattern of these genes in the Egyptian sheep breeds
Figure (1) represent agarose gel electrophoresis of PCR amplified FecB gene (190 pb) and AvaII digested FecB gene product of Rahmani (R), Ossimi (O), Awassi (A), Barki (B) and Awassi x Barki (A x B) breeds. It is clear from AvaII restriction fragment length polymorphism (RFLP) pattern represented in figure (1) that Digestion of FecB gene 190 base pair with Ava II restriction enzyme resulted in non carrier (++) 190 bp band in all the animals belonging to the five Egyptian breeds studied revealing absence of this restriction site of Ava II in those five Egyptian sheep breeds
Summary
Sheep contribute 6% of the total red meet produced in Egypt. The total sheep population in Egypt is 4,200,000 heads. Ossimi, and Barki, are of the main sheep breeds in Egypt with a population of 990,000, 514,000 , and 470,000 respectively (Galal et al, 2005).Regarding the economic importance of sheep in meat production in Egypt , it becomes essential to make fingerprinting of some genes related to economic traits such as litter size and growth rate in order to determine the polymorphism pattern of these genes in the Egyptian sheep breeds.
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