Abstract
Cnidium monnieri (L.) Cusson is a popular Traditional Chinese Medicine (TCM) with a variety of bioactivities. However, there are some problems that have affected the development of Cnidium monnieri (L.) Cusson. At present, many methods have been reported for the analysis of coumarins in Cnidium monnieri (L.) Cusson. However, the quality control of coumarins in Cnidium monnieri (L.) Cusson by high-speed counter-current chromatography (HSCCC) has not been reported. In this study, analytical high-speed counter-current chromatography (HSCCC) was successfully used for fingerprint of Cnidium monnieri (L.) Cusson with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at 4:6:6.5:3.5 (v/v). The UV wavelength was set at 254 nm. Six coumarin compounds with high biological activity were selected as indicator compounds for the quality control. The HSCCC fingerprint of the Cnidium monnieri (L.) Cusson was successfully established and there were some differences according to the results of the fingerprint analysis. The present results demonstrate that HSCCC is an established and efficient technique for the fingerprint analysis of Cnidium monnieri (L.) Cusson and can be used to control the quality of Cnidium monnieri (L.) Cusson. In brief, HSCCC is a useful technology for the fingerprint analytical method for TCM.
Highlights
Cnidium monnieri (L.) Cusson is a useful traditional Chinese medicine (TCM)
The results of the three extraction methods were as follows: It was revealed that 95% ethanol extraction was obviously superior to the traditional decoction method as shown in Table 1, in which the total contents of osthole and imperatorin of the total coumarins of Cnidium monnieri (L.) Cusson were nearly 10 times higher than those of the latter
The high-speed counter-current chromatography (HSCCC) method has only been applied in fingerprint studies on Salvia miltiorrhiza
Summary
Cnidium monnieri (L.) Cusson is a useful traditional Chinese medicine (TCM). It is located in all parts of China, and is distributed throughout the Russian Federation, North Korea, Vietnam, NorthAmerica, and some European countries. Coumarins are the principal active ingredients, including osthole (1), xanthotoxin (2), isopimpinellin (3), bergapten (4), and imperatorin (5) (Figure 1) [1]. These compounds are applied in various fields such as agriculture application: skilling worms and drying dampness; and medical application: relieving asthma, increasing sperm production, and expelling cold and rheumatism [2]. Different pharmacological activities such as antiallergic, antipruritic, antidermatophytic, antibacterial, antifungal, and antiosteoporotic activities have been found for these coumarins on the basis of modern pharmacological studies [3,4].
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