Abstract
Background: Cuscuta spp. known as dodders, have been used as traditional medicines in eastern and southern Asian countries for many disorders such as gastrointestinal, respiratory, endocrine, skin and neurological diseases (Drug of mania). Flavonoids are the main biologically active constituents in Cuscuta genus especially in C. chinensis. Our aim in this study was to identify and discriminate between C. chinensis samples which were collected from different hosts, by using the pattern recognition aided fingerprint analysis and comparison of the content of four major flavonoids (hyperoside, rutin, isorhamnetin, kaempferol). Material and methods: Samples were collected from nine different plants in the same time and place, dried, grinded and extracted with methanol (80%) by repeated maceration. Extractions were evaluated by high performance liquid chromatography analysis and fingerprinting. Results: Beside chromatographic fingerprint using similarity index, we compared the content of 4 major flavonoids (hyperoside, rutin, isorhamnetin, kaempferol) of C. chinensis samples on different hosts. Coclusion: The results showed that there were significant differences between the content of four major flavonoids of nine C. chinensis samples, but chromatographic fingerprint by similarity index of more than 0.88, showed that the sample consistency was similar. So, it was suggested that combination of chromatographic fingerprint and the content determination of major flavonoids could be used to evaluate the quality control of C. chinensis from different hosts.
Highlights
Cuscuta (Dodder) is a genus of about 100 - 170 species of yellow, orange or red parasitic plants which is treated as the specific genus in the family Cuscutaceae
This plant grows on different hosts but, it is said in Traditional Iranian Medicine (TIM) that C. chinensis which was used as herbal medicine has been collected from Alhagi maurorum. (Khar shotor) [12]
Flavonoids are the main content of C. chinensis, which indicates that we can suggest flavonoids contents of C. chinensis as its markers compounds for quality control and set a minimum qualification for C. chinensis with their major flavonoids like routin, isorhamnetin, hyperoside and kaempferol
Summary
Cuscuta (Dodder) is a genus of about 100 - 170 species of yellow, orange or red (rarely green) parasitic plants which is treated as the specific genus in the family Cuscutaceae. Chromatographic fingerprint and contents of the markers were applied for quality control of C. chinensis samples This plant grows on different hosts but, it is said in Traditional Iranian Medicine (TIM) that C. chinensis (koshoos) which was used as herbal medicine has been collected from Alhagi maurorum. Our aim in this study was to identify and discriminate between C. chinensis samples which were collected from different hosts, by using the pattern recognition aided fingerprint analysis and comparison of the content of four major flavonoids (hyperoside, rutin, isorhamnetin, kaempferol). Results: Beside chromatographic fingerprint using similarity index, we compared the content of 4 major flavonoids (hyperoside, rutin, isorhamnetin, kaempferol) of C. chinensis samples on different hosts. It was suggested that combination of chromatographic fingerprint and the content determination of major flavonoids could be used to evaluate the quality control of C. chinensis from different hosts
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