Abstract
Polymorphonuclear leukocytes (PMN's) derived from the peritoneal cavity of guinea pigs or rabbits after stimulation by sodium caseinate showed firm antibody-mediated adherence to the cuticle of secondand third-stage larvae of Ascaris suum. The fine structural features of such cells were similar to those reported by other investigators. Wrinkling and dissolution of the cuticle was observed in areas of leukocyte-parasite contact. Depletion of specific granules of the attached cells was often evident in these areas of contact. In a previous report (Morseth and Soulsby, 1969), the fine structure details of pyroninophils (immunoblasts) showing antibody-mediated adherence to the surface of Ascaris suum larvae were described. The adhesion in this system occurs when lymphocytes, stimulated to transform by antigen, are incubated with larvae sensitized by antibody derived from animals immune to A. suum. Complement is not required to effect adhesion of pyroninophils (Soulsby, 1967a). The requirements for antibody-mediated adherence of polymorphonuclear leukocytes (PMN's) to A. suum larvae are distinctly different. In this system PMN's from either immune or normal animals are reactive and they may be derived from a different species to that supplying the immune serum. The serum possibly acts as an opsonin and complement is required for adhesion to occur (Soulsby, 1967a). The present paper deals with fine structural aspects of PMN's, caused to adhere to the cuticle of ascaris larvae, and the cuticular alterations associated with these adhering cells. This study is part of a wider investigation of the formation of parasitic granulomata, especially the sequence of cell types infiltrating the area and concomitant cellular events associated with the granuloma formation. Immunological details of PMN adherence to larvae will be published elsewhere. Received for publication 11 February 1969. * Work supported by American Cancer Society Institutional Grant IN-38 (D.J.M.), USPHS General Research Support Grant IS01 FR-05464 (D.J.M.), and USPHS Research Grant AI-06262 E.J.L.S.). MATERIALS AND METHODS
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