Abstract
Summary. In rabbit spermatozoa, acid phosphatase activity is primarily localized within unique, morphologically distinct bands beneath the equatorial segment of the acrosome; secondary localizations appear along the entire postacrosomal segment of the sperm head. In bull spermatozoa, acid phosphatase activity is associated with the anterior third of the postacrosomal segment. The enzyme was extractable with detergent after a pretreatment with pyrimidinetrione (barbituric acid). Activity was inhibited by sodium fluoride and destroyed by fixation. The localization of acid phosphatase in the postacrosomal segment of both species and the unique localizations in the rabbit equatorial segment may ultimately relate to sperm penetration or gamete membrane fusion mechanisms active during the fertilization process.
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