Fine structure, Histochemistry, and Morphogenesis During Excystment of the Podocysts of the Giant Jellyfish Nemopilema nomurai (Scyphozoa, Rhizostomeae)

Abstract

Production of podocysts is the exclusive form of asexual reproduction by polyps of the giant jellyfish Nemopilema nomurai, which has been recurrently blooming in the East Asian seas in the last decade. Podocycts consist of a dome-shaped chitinous capsule with laminated structure that encapsulates a mass of cyst cells filled with granules containing nutrient reserves such as proteins, carbohydrates, and lipids. Mitochondria, rough endoplasmic reticulum, and Golgi complexes are scarce in the cytoplasm of these cells, and the staining reaction for RNA is weak, indicating very low metabolic activity. Podocysts are capable of dormancy for at least 5 years without significant change of internal structure or nutrient reserves. Integrated information about spontaneous and artificially induced metamorphosis suggests that the following processes occur during excystment: (1) nematocyst formation in the internal cell mass, (2) stratification of the cell mass into endoderm and ectoderm, (3) extrusion of the cell mass through a gradual opening of the capsule, (4) formation of primordial polyp mouth and tentacles, and (5) metamorphosis to a polyp. We morphologically confirmed that N. nomurai podocysts have the capacity for long-term dormancy, an ability that should contribute to the periodic nature of the massive blooms of medusae of this species.