Fine structure and elemental analysis of the enamel in the cryptobranchid salamander Megalobatrachus japonicus.

Abstract

Teeth from Megalobatrachus japonicus were prepared for scanning electron microscopy (SEM) and electron microprobe analyzer (EMPA) to assay the elements of the enamel layer. The enamel layer of each of tooth is thin and the arrangement of the enamel crystals is regular in the Megalobatrachus japonicus. Based on calcium, phosphate and magnesium levels of distribution, the calcification level of the enamel layer differs between the labial and lingual surfaces. Concentrations of iron and fluoride are located in both portions of the enamel layer as well. The presence of trace elements and distribution of calcified levels are related to the mineral formation in the enamel layer and function of the teeth during feeding.