Abstract
The sub-cellular localization of acid phosphatase and peroxidase activities has been studied by cytochemical procedures in cells at the surface of the separation layer during the abscission of leaves of Phaseolus vulgaris. Intense staining for both enzymes was found in the cell walls, Golgi bodies and endoplasmic reticulum. The wall staining for acid phosphatase was chiefly associated with the middle lamellar region while staining for peroxidase was found throughout the wall. These observations are discussed in relation to the possible involvement of these enzymes in the changes occurring in the wall during abscission and to the role of the Golgi bodies in the separation process.
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