Abstract
Digitonin was incorporated into tissue fixation to localize free cholesterol during fat absorption by the bovine small intestine. Reaction products were associated with lipid droplets in the apical part of epithelial cells and, to a limited extent, with lipid-containing regions of the Golgi apparatus and intercellular spaces. These observations suggest that cholesterol is associated with lipid droplets early in the transport phase and that such an association may be the result of the process of chylomicron formation.
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