Abstract
BackgroundCyclical parthenogenetic water fleas of the genus Daphnia have become a prominent model organism in ecology and evolution. In the past, analyses of their population structure have been limited by the prevailing use of allozyme markers, which in general do not allow for the distinction of individual clones. In this study, we used 10 microsatellite markers to track changes in the taxonomic and clonal composition of Daphnia populations, and traced the abundance of the most common clones in two European reservoirs. One of the localities was inhabited by a single species of the Daphnia longispina complex (D. galeata), the other by two parental species (D. galeata and D. longispina) and their interspecific hybrids. The study took place during the transition from summer stratification to autumn mixing, representing a period of major environmental change within lake habitats.ResultsIn both reservoirs, we observed temporal (generation-to-generation) and spatial (along the heterogeneous reservoir environment) changes in Daphnia community structure. In the single-species reservoir, the clonal diversity of D. galeata increased with time, as a few dominant clones were replaced by a higher number of less common clones. A loss in selective advantage for the dominant clones may have been due to gradual changes in the environment, or due to selection acting in a negative frequency-dependent manner. In the multispecies reservoir, there were no apparent temporal trends in clonal diversity but we observed significantly lower clonal diversity in the interspecific hybrids than in the coexisting parental species, supporting the existence of reproductive barriers between the parental genomes.ConclusionsOur study, tracing clonal lineages of Daphnia in time and space by the fine-resolution markers, contributes to the understanding of how clonal reproduction impacts community structure in cyclically parthenogenetic organisms.
Highlights
Cyclical parthenogenetic water fleas of the genus Daphnia have become a prominent model organism in ecology and evolution
Hybridization has been documented within several species complexes of Daphnia from Eurasia, North America and Australia [12,13], but most research has concentrated on the D. longispina complex, inhabiting permanent lakes of the northern temperature zone [13]
Some of these clustered around the reference clones of D. galeata, the others were positioned in between the clusters representing two parental species: D. galeata and D. longispina (Figure 1b)
Summary
Cyclical parthenogenetic water fleas of the genus Daphnia have become a prominent model organism in ecology and evolution. Parthenogenetic organisms, which reproduce both sexually and asexually, are common in nature, in both the animal and plant kingdoms [1,2]. This mode of reproduction, especially its asexual (clonal) component, has attracted considerable interests among population geneticists and evolutionary biologists. In Europe, this complex includes, together with some rarer taxa, the widespread and ecologically important species D. cucullata, D. galeata and D. longispina [14] These species often form interspecific hybrids which sometimes reach high abundances [13,15,16,17,18]. In the D. longispina complex, parental species reproduce clonally for most of the year, there is evidence that they invest more into sexual reproduction than their F1 hybrids [16,20]
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