Abstract

Publisher Summary This chapter explains the way additional related loci can be searched for near CYP4All, genes and pseudogenes can be identified, and the genomic sequence assembly currently available in the public database can be evaluated. This general method can be adapted to the fine-scale mapping of any P450 gene cluster in any organism for that there is a substantial collection of genomic sequence available through GenBank. It relies throughout on data and software that are freely available to all researchers. Researchers can also continue to monitor a chromosomal region and incorporate improved sequences into their provisional maps. A small region that does not contain any repetitive element sequence can be selected from the end of a clone flanking a gap and used to search the database periodically for new, potentially overlapping sequences. Searches using cDNA sequences from unlocalized genes can also be done periodically. In this case, the true CYP4All locus is still missing.

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