Abstract
The quantitative trait locus (QTL) qBlsr5a on the short arm of rice ( Oryza sativa L.) chromosome 5 has been proved to have the largest effect on the resistance to rice bacterial leaf streak ( Xanthomonas oryzae pv. oryzicola, BLS). Using Acc8558 (highly resistant to BLS) as the donor and H359 (highly susceptible to BLS) as the recipient, a near-isogenic line (NIL) H359-BLSR5a was developed through backcross and only qBlsr5a was introgressed from the donor parent. A big F 2 population (2,265 individuals) was constructed by hybridizing the NILs with H359 and 120 individuals with extreme phenotypes (lesion length < 2 cm) were selected. Eighty-five out of the 120 individuals were identified as homozygous resistant genotypes at the target QTL after examining their progeny lines (F 2:3). By genotyping these homozygous individuals with SSR markers and performing linkage analysis, qBlsr5a was mapped to an interval between SSR markers RM153 and RM159, which covered a range of 2.4 cM or 290 kb.
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