Abstract
The cuticular wax covering epidermal cells causes the glaucous appearance in cabbage. As a protective barrier, cuticular wax plays various roles in protection against biotic and abiotic stresses. This is the first gene mapping report of a dominant glossy green cabbage mutant. In the present paper, scanning electron microscopy (SEM) demonstrated that the wax crystals were severely reduced in the mutant, which indicates that the glossy green phenotype is caused by cuticular wax reduction. Genetic analysis revealed that the glossy trait is controlled by a single dominant gene. Through primer screening and fine mapping, the mutant gene BoGL1 (Brassica oleracea glossy 1) was delimited to the end of chromosome C08 by the flanking marker SSRC08–76 at a genetic distance of 0.2 cM. Two genes homologous to CER1 (ECERIFERUM 1), a gene related to wax biosynthesis in Arabidopsis, were located in the mapped region. Expressional analysis revealed that the Bol018504 gene was severely suppressed but that no nucleotide variation was found by sequencing. These results lay the foundation for the functional analysis of BoGL1, and they will accelerate the research on wax metabolism in cabbage.
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