Abstract
Rice tiller angle is an important agronomic trait that contributes to crop production and plays a vital role in high yield breeding. In this study, a recombinant inbred line (RIL) population derived from the cross of a glabrous tropical japonica rice D50 and an indica rice HB277, was used to investigate quantitative trait loci (QTLs) controlling rice tiller angle. Two major QTLs, qTAC8 and qTAC9, were detected. While qTAC9 mapped with a previously identified gene (TAC1), using a BC2F2 population qTAC8 was mapped to a 16.5 cM region between markers RM7049 and RM23175. Position of qTAC8 was narrowed to a 92 kb DNA region by two genetic segregating populations. Finally, one opening reading frame (ORF) was regarded as a candidate gene according to genomic sequencing and qRT-PCR analysis. In addition, a set of four near isogenic lines (NILs) were created to investigate the genetic relationship between those two QTLs, and one line carrying qTAC8 and qTAC9 presented additive effect of tiller angle, suggesting that these QTLs are involved in different genetic pathways. Our results provide a foundation for the cloning of qTAC8 and genetic improvement of the rice plant architecture.
Highlights
Rice (Oryza sativa L.) is one of the most important food crops in China and the world
Compact plants prejudice in capturing light and prevention of plant diseases and insect pests, appropriate tiller angle is beneficial for improving rice production [3, 4]
QTL mapping strategy was conducted and two major QTLs for tiller angle named qTAC8 on chromosome 8 and qTAC9 on chromosome 9 were identified in this recombinant inbred line (RIL) population. qTAC8 and qTAC9 were mapped within the region of RM339-RM210 and RM201-RM7306, respectively. qTAC8 could account for almost 33.4% of the variance, while qTAC9 could explain around 17.4% of the variance in tiller angle (Table 1)
Summary
Rice (Oryza sativa L.) is one of the most important food crops in China and the world. It is the most effective safeguard for food security and agricultural sustainable development through high-yielding rice breeding. The angle between the main culm and its side tillers [1], is a decisive factor for building ideal plant architecture, whereby neither spreadout rice nor compact type rice is beneficial for grain production [2]. Compact plants prejudice in capturing light and prevention of plant diseases and insect pests, appropriate tiller angle is beneficial for improving rice production [3, 4].
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