Abstract
The efficiency of hybrid seed production can be improved by increasing the percentage of exserted stigma, which is closely related to the stigma length in rice. In the chromosome segment substitute line (CSSL) population derived from Nipponbare (recipient) and Kasalath (donor), a single CSSL (SSSL14) was found to show a longer stigma length than that of Nipponbare. The difference in stigma length between Nipponbare and SSSL14 was controlled by one locus (qSTL3). Using 7,917 individuals from the SSSL14/Nipponbare F2 population, the qSTL3 locus was delimited to a 19.8-kb region in the middle of the short arm of chromosome 3. Within the 19.8-kb chromosome region, three annotated genes (LOC_Os03g14850, LOC_Os03g14860 and LOC_Os03g14880) were found in the rice genome annotation database. According to gene sequence alignments in LOC_Os03g14850, a transition of G (Nipponbare) to A (Kasalath) was detected at the 474-bp site in CDS. The transition created a stop codon, leading to a deletion of 28 amino acids in the deduced peptide sequence in Kasalath. A T-DNA insertion mutant (05Z11CN28) of LOC_Os03g14850 showed a longer stigma length than that of wild type (Zhonghua 11), validating that LOC_Os03g14850 is the gene controlling stigma length. However, the Kasalath allele of LOC_Os03g14850 is unique because all of the alleles were the same as that of Nipponbare at the 474-bp site in the CDS of LOC_Os03g14850 among the investigated accessions with different stigma lengths. A gene-specific InDel marker LQ30 was developed for improving stigma length during rice hybrid breeding by marker-assisted selection.
Highlights
Rice (Oryza sativa L.) is a main cereal crop for billions of people worldwide
Whereas indica hybrid rice accounts for 80% of the planting area of indica rice, japonica hybrid rice accounts for only 3% of the planting area of japonica rice [1]
The stigma length of SSSL14 was significantly longer than that of Nipponbare (i.e., 1.93±0.04 vs. 1.71±0.02 mm) in 2013, which was consistent with the measurements collected in 2012
Summary
Rice (Oryza sativa L.) is a main cereal crop for billions of people worldwide. In China, the rice planting area is approximately 3.2×107 ha each year, and hybrid rice is planted in over half of the total rice growing area. Whereas indica hybrid rice accounts for 80% of the planting area of indica rice, japonica hybrid rice accounts for only 3% of the planting area of japonica rice [1]. Fine Mapping and Candidate Gene Analysis of qSTL3 in Rice. A main limiting factor hindering the extension of the japonica hybrid rice area is the low yield of hybrid seed production. A low yield of F1 seed production is mainly caused by a low outcrossing rate of the maternal parent (CMS lines or TGMS lines) in the F1 seed production field
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