Abstract
The aim of this study was to evaluate the tissue compatibility of a silorane-based resin system (Filtek™ Silorane) and a methacrylate-based nanoparticle resin (Filtek™ Supreme XT) after implantation in the subcutaneous connective tissue of isogenic mice. One hundred and thirty five male isogenic BALB/c mice were randomly assigned to 12 experimental and 3 control groups, according to the implanted material and the experimental period of 7, 21 and 63 days. At the end of each period, the animals were killed and the tubes with the surrounding tissues were removed and processed for microscopic analysis. Samples were subjected to a descriptive and a semi-quantitative analyses using a 4-point scoring system (0-3) to evaluate the collagen fiber formation and inflammatory infiltrate. Data were statistically analyzed using the Kruskal Wallis test (α=0.05). The results showed that there was no significant difference between the experimental and control groups considering the three evaluation periods (p>0.05). The silorane-based and the methacrylate-based nanoparticle resins presented similar tissue response to that of the empty tube (control group) after subcutaneous implantation in isogenic mice.
Highlights
Methacrylate-based composite resins release uncured monomers to the tissues [1], which have been associated with hypersensitivity and cytotoxicity [2], genotoxicity [3], estrogenicity [4], and immune system alterations [5].Cell culture studies have demonstrated that methacrylate and dimethacrylate monomers, commonly used in the restorative polymeric technology, may affect the recruitment of leukocytes in inflammation sites by decreasing the expression of intercellular adhesion molecules and inducing enzymatic activity and expression of growth factors and cellular cytokines [6]
The present study evaluated the tissue compatibility of a silorane-based resin system (FiltekTM Silorane) and a methacrylate-based nanoparticle resin (FiltekTM Supreme XT) after implantation in the subcutaneous connective tissue of isogenic mice
A 1-cm-wide incision was made on the dorsal region followed by tissue divulsion with Kelly forceps, and the tube was inserted into the connective tissue and the skin borders were closed with 4-0 silk sutures (Vicryl; Johnson & Johnson: Ethicon Inc., New Brunswick, NJ, USA)
Summary
Methacrylate-based composite resins release uncured monomers to the tissues [1], which have been associated with hypersensitivity and cytotoxicity [2], genotoxicity [3], estrogenicity [4], and immune system alterations [5].Cell culture studies have demonstrated that methacrylate and dimethacrylate monomers, commonly used in the restorative polymeric technology, may affect the recruitment of leukocytes in inflammation sites by decreasing the expression of intercellular adhesion molecules and inducing enzymatic activity and expression of growth factors and cellular cytokines [6]. Methacrylate-based composite resins release uncured monomers to the tissues [1], which have been associated with hypersensitivity and cytotoxicity [2], genotoxicity [3], estrogenicity [4], and immune system alterations [5]. Resin monomers suppress the mitochondrial activity of macrophages and alter their inflammatory responses [4,7]. In this way, reactive components released from conventional composite resins, such as unreacted monomers [1] and oxidation products, may induce toxicity or inflammatory tissue reactions [8]. Though adequate physical properties have been attributed to these resins (e.g.: improved surface polishing) [10], they have shown toxic effects in cell cultures [1]
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