Filling the gap: Micro-C accesses the nucleosomal fiber at 100-1000 bp resolution.

Julien Mozziconacci,Romain Koszul

doi:10.1186/s13059-015-0744-8

Abstract

The fine three-dimensional structure of the nucleosomal fiber has remained elusive to genome-wide chromosome conformation capture (3C) approaches. A new study mapping contacts at the single nucleosome level (Micro-C) reveals topological interacting domains along budding yeast chromosomes. These domains encompass one to five consecutive genes and are delimited by highly active promoters.

Highlights

Over the past ten years, genome-wide derivatives of the chromosome conformation capture approach (3C [1] and Hi-C [2]) have provided important mechanical and functional insights into the organizational principles of eukaryotic and prokaryotic genomes
* Correspondence: mozziconacci@lptmc.jussieu.fr; romain.koszul@pasteur.fr 1Theoretical Physics for Condensed Matter Laboratory, UPMC, CNRS UMR 7600, Sorbonne Universités, Place Jussieu, 75005 Paris, France 2Institut Pasteur, Department Genomes and Genetics, Groupe Régulation Spatiale des Génomes, 75015 Paris, France Full list of author information is available at the end of the article a micrococcal nuclease (Mnase) chromosome conformation assay dubbed Micro-C, in yeast Saccharomyces cerevisiae
Micro-C reveals multi-gene domains in S. cerevisiae The Micro-C approach developed by Rando and colleagues [5] elegantly alleviates some of the Chromosome conformation capture (3C) limitations by exploiting the regular spacing of nucleosomes (167 bp in yeast) along the DNA strand

Summary

Introduction

Over the past ten years, genome-wide derivatives of the chromosome conformation capture approach (3C [1] and Hi-C [2]) have provided important mechanical and functional insights into the organizational principles of eukaryotic and prokaryotic genomes. * Correspondence: mozziconacci@lptmc.jussieu.fr; romain.koszul@pasteur.fr 1Theoretical Physics for Condensed Matter Laboratory, UPMC, CNRS UMR 7600, Sorbonne Universités, Place Jussieu, 75005 Paris, France 2Institut Pasteur, Department Genomes and Genetics, Groupe Régulation Spatiale des Génomes, 75015 Paris, France Full list of author information is available at the end of the article a micrococcal nuclease (Mnase) chromosome conformation assay dubbed Micro-C, in yeast Saccharomyces cerevisiae. Micro-C reveals multi-gene domains in S. cerevisiae The Micro-C approach developed by Rando and colleagues [5] elegantly alleviates some of the 3C limitations by exploiting the regular spacing of nucleosomes (167 bp in yeast) along the DNA strand.

Results

Conclusion