Filamentous sulfur-oxidizing bacteria of the genus Thioploca from Lake Tonle Sap in Cambodia

Abstract

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 66:295-300 (2012) - DOI: https://doi.org/10.3354/ame01578 NOTE Filamentous sulfur-oxidizing bacteria of the genus Thioploca from Lake Tonle Sap in Cambodia Fumiko Nemoto1, Hisaya Kojima1,*, Akifumi Ohtaka2, Manabu Fukui1 1The Institute of Low Temperature Science, Hokkaido University, Kita-19, Nishi-8, Kita-ku, Sapporo 060-0819, Japan 2Faculty of Education, Hirosaki University, 1-Bunkyocho, Hirosaki, Aomori 036-8560, Japan *Corresponding author. Email: kojimah@pop.lowtem.hokudai.ac.jp ABSTRACT: Members of the genus Thioploca are uncultured filamentous sulfur-oxidizing bacteria that live in freshwater/brackish sediments and have the ability to store nitrate in high concentrations in their cells. Their close relatives that inhabit marine sediments, such as Thiomargarita and ‘Candidatus Marithioploca’, are thought to greatly influence cycles of sulfur, nitrogen, and phosphorus. To date, the genus Thioploca has been reported only from temperate and subarctic areas. Our demonstration of Thioploca in Lake Tonle Sap, Cambodia, is the first report of this genus in a tropical lake. The filaments obtained from Lake Tonle Sap were morphologically similar to those of other lakes. Phylogenetic analysis based on genes for 16S rRNA, 23S rRNA, and the 16S-23S rRNA internal transcribed spacer (ITS) region revealed that 3 distinct lineages coexist in this lake. These results indicate that the geographical distribution and phylogenetic diversity of the genus Thioploca is greater than previously thought. KEY WORDS: Sulfur-oxidizing bacteria · Freshwater lake · Sediment · Phylogeny Full text in pdf format PreviousCite this article as: Nemoto F, Kojima H, Ohtaka A, Fukui M (2012) Filamentous sulfur-oxidizing bacteria of the genus Thioploca from Lake Tonle Sap in Cambodia. Aquat Microb Ecol 66:295-300. https://doi.org/10.3354/ame01578 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 66, No. 3. Online publication date: July 09, 2012 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2012 Inter-Research.