Abstract
Olive mill wastewaters and olive cake are effluents generated during olive oil production process. They represent a major disposal and potentially severe pollution problem for the industry, also promising source of substances of high value. The aim of this study is the valorization of olive mill wastes (OMWW, olive cake, olive twigs and leaves) to produce enzymes with high industrial and biotechnological potential, by the solid-state fermentation technique (SSF), from isolated fungi present in olive mill wastewater and olive cake. A total of 47 strains were isolated and purified from these two residues. The metabolic potential of isolated strains was study by testing the hydrolytic enzymes activities of lipase, protease, amylase, cellulase, invertase, phytase and tannase on agar plate media containing different substrate. The monitoring of SSF has shown that the metabolic activity of these strains is extremely rapid using this technique. Our fungi collection contains a diversity of strains capable to producing a variety of enzymes of biotechnological interest.
Highlights
Food industries produce a large volume of solid and liquid residues, which represent disposal and potential environmental pollution problem (Fernández-Bolaños et al, 2006)
This study aims to develop an innovative process for the valorization of olive oil wastes (OMWW, olive cake (OC) and olive twigs and leaves (OTL)
Fungal isolates During the 2015-2016 olive oil production campaign, 40 olive mills waste water and olive cake samples were taken from different olive mills
Summary
Food industries produce a large volume of solid and liquid residues, which represent disposal and potential environmental pollution problem (Fernández-Bolaños et al, 2006). Olive oil production, one of the foremost agro-food industries in Mediterranean countries such as Tunisia. The manufacturing process of olive oil production yields enormous quantities of two byproducts depending on the production system adopted: olive cake (OC) and olive mill wastewater (OMWW) (Nefzaoui, 1995). These two wastes are hazardous to the environment; mainly for their high organic load and chemical composition that renders them resistant to degradation (Cegarra et al, 2006; Morillo et al, 2009). Received in revised form: 26 Nov 2020. The journal will continue to appear quarterly, as before, with four annual numbers
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