Filaggrin genotype does not determine the skin's threshold to UV-induced erythema

Deborah Forbes,Leona Johnston,June Gardner,Stephanie F Maccallum,Linda E Campbell,Albena T Dinkova-Kostova,W.H Irwin Mclean,Sally H Ibbotson,Robert S Dawe,Sara J Brown

doi:10.1016/j.jaci.2015.11.022

Deborah Forbes, Leona Johnston + Show 8 more

Open Access

https://doi.org/10.1016/j.jaci.2015.11.022

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Abstract

Filaggrin genotype does not determine the skin's threshold to UV-induced erythema

Highlights

This study aimed to evaluate the expression level of epidermal proteins by using a tape stripping method to determine whether these proteins can be used as biomarkers predictive of atopic dermatitis (AD) development in infants
Exclusion criteria were history of skin allergy, eczema/AD, psoriasis or polymorphic light eruption, immunosuppression caused by medications or disease, photosensitizing medication, and a holiday abroad or sunbed use within the preceding 4 weeks
Detailed phototesting was undertaken by using an irradiation monochromator, which is a diffraction grating device with a 1.6-kW or 450-W xenon arc lamp.E2,E3 This instrument allows irradiation of small areas of the skin over a range of wavebands, which are included in the solar spectrum (UVB to UVA/visible)

Summary

Assessment of MEDs of different wavebands in healthy volunteers

Healthy adults with clinically normal skin of phototypes I to IIIE1 who had previously participated in research studies within the National Photobiology Unit (Dundee, Scotland, United Kingdom) were invited to participate in this study. Detailed phototesting was undertaken by using an irradiation monochromator, which is a diffraction grating device with a 1.6-kW or 450-W xenon arc lamp.E2,E3 This instrument allows irradiation of small areas of the skin over a range of wavebands, which are included in the solar spectrum (UVB to UVA/visible). On day 1, approximately 1-cm[2] areas of the skin on the volunteer’s back were exposed to UV and visible light according to a standardized procedure established in the National Photobiology Unit. The irradiation procedure was repeated on day 2 on a separate area of back skin using smaller dose increments (10% to 20%) across a narrower range of doses at each waveband selected on the basis of the MEDs seen 24 hours after first irradiation to establish the MED precisely.

Power calculation

Findings

Statistical analysis of monochromator phototesting data