FI-HG-AAS를 이용한 전처리 과정에서 사용되는 예비환원제의 종류에 따른 요중 비소 분석결과 비교

Abstract

*Institute for Occupational & Environmental Health, Korea University, Seoul, Republic of korea**Graduate School of Public Health, Yonsei University, Seoul, Republic of KoreaABSTRACTObjectives: The method of analyzing urinary arsenic by flow injection hydride generation atomicabsorption spectrometry (FI-HG-AAS) is generally used because it shows relatively greater sensitivity, lowdetection limits, low blocking action, and is simple to operate. In this study, the results of analysisaccording to three pre-reductants commonly used in the FI-HG-AAS method were compared with eachother. Methods: To analyze urinary arsenic, nineteen urine samples were collected from adults aged 43-79 years oldwithout occupational arsenic exposure. Analysis equipment was FI-HG-AAS (AAnalyst 800/FIAS 400, Perkin-Elmer Inc., USA). The three pre-reductants were potassium iodide (KI/AA), C3H7NO2S (L-cysteine), and amixture of KI/AA and L-cysteine (KI/AAL 74.96µg/L for L-cysteine; 69.23µg/L for KI/AA and L-cysteine; 13.06µg/L for KI/AA) and were significantlycorrelated (R2=0.882). In addition, they showed the lowest coefficient of variation in the results between twolaboratories that applied the same method.Conclusion: The efficiency of hydride generation is considered highly important to the analysis of urinaryarsenic via FI-HG-AAS. This study suggests that using L-cysteine as a pre-reductant may be suitable and themost rational among the FI-Hg-AAS methods using pre-reductants. Keywords: Hydride Generation atomic absorption spectrometry, KI/ascorbic acid, L-cysteine, Pre-reductant,Urinary arsenic