Figure S4 from Antiangiogenic Tyrosine Kinase Inhibitors have Differential Efficacy in Clear Cell Renal Cell Carcinoma in Bone

Abstract

<p>Effects of TKIs on blood vessels in RENCA VHL- bone and lung tumors. A) Visual representation of the experimental approach. B,C) Bone tumors: representative pictures captured at the confocal microscope and quantification of blood vessel area in tumor (green, GFP), nuclei (blue, DAPI); blood vessels (yellow, endomucin; red, laminin); dotted line, tumor edge; single channel endomucin is shown. A quantification of % blood vessels on tumor area is shown, mean + SEM, n=3-4/group; Bar, 100 µm. D,E) Lung tumors: representative pictures captured at the confocal microscope and quantification (C) of blood vessel area in tumor (green, GFP), nuclei (blue, DAPI); blood vessels (red, CD31); dotted line, tumor edge; single channel CD31 is shown. A quantification of % blood vessels on tumor area is shown, mean + SEM, n=3-4/group; Bar, 100 µm P values by one-way ANOVA with Tukey’s HSD post hoc test; p < 0.05 = *, p < 0.01 = **. BV- blood vessels. Endo-endomucin; Lam-laminin; BV-blood vessels; V-vehicle, A-axitinib, C-cabozantinib, L-lenvatinib;</p>