Figure S3 from Interleukin-15 Enhances Anti-GD2 Antibody-Mediated Cytotoxicity in an Orthotopic PDX Model of Neuroblastoma

Abstract

<p>Supplementary Figure S3. NK cell ligands in SJNBL46_X passaged in NOD scid Il2rγâ^'/â^' (NSG) and nude mice. A, SJNBL46_X grew in NSG mice and was subsequently analyzed for the expression of NK cell ligands by flow cytometry and reinjected into nude mice. Forming tumors in nude mice underwent the same analysis. Histograms are shown for each ligand (red: NSG mouse, blue: nude mouse, black: isotype). B, The opposite experiment was performed, in which PDX was harvested from nude mice and reinjected into NSG mice. Histograms depict NK cell ligand expression in these tumors (red: nude mouse, blue: NSG mouse, black: isotype). The analysis showed that there were NK cell ligands that did not change expression when tumors from NSG mice were passaged through nude mice (e.g., ULBP2, DR4, CD112, MICA, and MICB) or vice versa (e.g., DR4, DR5, CD112, ULBP3, MICA, and MICB). There were other ligands that were downregulated. However, downregulation was noted in both sub-sets of experiments (e.g., NTB-A and CD155), suggesting that this was not due to host-related immune editing.</p>