Figure S2 from Itraconazole-Induced Inhibition on Human Esophageal Cancer Cell Growth Requires AMPK Activation

Abstract

<p>Figure S2. TE-1 cells, expressing AMPKα1 shRNA ("sh-AMPKα1") or scramble control shRNA ("shRNA-Ctrl"), as well as the parental control TE-1 cells ("No shRNA"), were treated with itraconazole ("Itra", 3.0 μg/mL) for indicated time, and were tested by Western blot of listed proteins (A and B); Cell viability (MTT assay, C) and cell death (trypan blue staining assay, D) were tested. The primary human esophageal cancer cells ("Primary cancer cells") or HEEC esophageal epithelial cells were treated with/without itraconazole ("Itra", 3.0 μg/mL) for applied time, and were tested of listed proteins (E-G). Notably, RTKs (EGFR, PDGFRα and PDGFRβ) expression and Akt activation were low in HEEC cells. # P < 0.05 vs. group "shRNA-Ctrl".</p>