Figure S1 - S14 from Super-Enhancer–Associated Long Noncoding RNA HCCL5 Is Activated by ZEB1 and Promotes the Malignancy of Hepatocellular Carcinoma

Abstract

<p>Supplementary Figure 1. Screening and Cloning of lncRNA HCCL5 in hepatocellular carcinoma. Supplementary Figure 2. Subcellular localization of HCCL5 in SMMC-7721 cells. Supplementary Figure 3. The mRNA expression of HCCL5 was measured across different HCC and nonmalignant cell lines. Supplementary Figure 4. The expression of HCCL5 was measured by qRT-PCR upon either over-expression or knockdown of HCCL5. Supplementary Figure 5. HCCL5 accelerated cell proliferation of HCC cells in vitro. Supplementary Figure 6. HCCL5 accelerated cell cycle in HCC cells. Supplementary Figure 7. HCCL5 overexpression had no effect on cell death of HCC cells. Supplementary Figure 8. HCCL5 interference had no effect on cell death of HCC cells. Supplementary Figure 9. HCCL5 deteriorated cell migration and invasion of HCC cells in vitro. Supplementary Figure 10. HCCL5 promotes xenogeneic growth while inhibits apoptosis of HCC cells in vivo. Supplementary Figure 11. The protein levels of EMT markers in HCC cells stably expressing HCCL5 overexpression or shRNA vectors. Supplementary Figure 12. Survival analysis of E-cadherin and Vimentin expression in TCGA HCC patients. Supplementary Figure 13. The protein level of Twist in HCC cells stably expressing HCCL5 shRNA vectors. Supplementary Figure 14. The luciferase activities of five constituent enhancers (E1-E5) within the super-enhancer of HCCL5 in 293T cells.</p>