FIGURE 5 from Metastatic Competency and Tumor Spheroid Formation Are Independent Cell States Governed by RB in Lung Adenocarcinoma

Abstract

<p>RB restoration reverses metastatic competency. <b>A,</b> Contingency analysis of the number of mice injected with single-cell suspensions produced from Group 1 tumor spheroids and treated with tamoxifen (<i>Rb<sup>R</sup><sup>/R</sup></i>) or vehicle (<i>Rb<sup>TR</sup><sup>/TR</sup></i>). Outcome of interest is tumor formation 5 weeks after injection. Statistical significance was assessed by <i>χ</i><sup>2</sup> test. For <i>KP;Rb<sup>TR</sup><sup>/TR</sup></i> mice, <i>n</i> = 9, and for <i>KP;Rb<sup>R</sup><sup>/R</sup></i> mice, <i>n</i> = 8. <b>B,</b> Number of tumors forming in mice five weeks after injection with single-cell suspensions produced from Group 1 tumor spheroids. Statistical significance assessed by Student <i>t</i> test. For <i>KP;Rb<sup>TR</sup><sup>/TR</sup></i> mice, <i>n</i> = 9, and for <i>KP;Rb<sup>R</sup><sup>/R</sup></i> mice, <i>n</i> = 8. Error bars represent mean ± SD. <b>C,</b> Lung mass in mice 5 weeks after injection with single-cell suspensions produced from Group 1 tumor spheroids. Statistical significance assessed by Student <i>t</i> test. For <i>KP;Rb<sup>TR</sup><sup>/TR</sup></i> mice, <i>n</i> = 9, and for <i>KP;Rb<sup>R</sup><sup>/R</sup></i> mice, <i>n</i> = 8. Error bars represent mean ± SD. <b>D,</b> Representative lung sections stained with hematoxylin and eosin from mice injected with single-cell suspensions produced from Group 1 tumor spheroids. Lung section images were taken with a Leica DMI6000 using a 5X objective.</p>