FIGURE 4 from Chromatin Accessibility Landscape of Human Triple-negative Breast Cancer Cell Lines Reveals Variation by Patient Donor Ancestry

Abstract

<p>Hypoxia exacerbates donor ancestry-related differences in TF activity in cultured TNBC cell lines. <b>A,</b> Chromosome map showing the distribution of DARs between EA and AA TNBC cells when subjected to hypoxic conditions (FC > 2, FDR < 0.01). <b>B,</b> Differential digital footprinting in EA- and AA-derived TNBC lines was performed using TOBIAS to identify open chromatin regions and their TF binding sites. Shown are the top AA- or EA-associated TFs whose predicted binding activity increased by a defined magnitude of >0.05 under hypoxia. PPI network of TFs with differential binding capacities (DBS > 22.5, FDR < 0.01) that are up in EA (<b>C</b>) and AA (<b>D</b>) under hypoxia using the STRING database. Top 15 significantly enriched pathways (FDR < 0.01, Reactome-based) based on a TF network analysis.</p>