Figure 3 from FUME-TCRseq Enables Sensitive and Accurate Sequencing of the T-cell Receptor from Limited Input of Degraded RNA

Abstract

<p><b>A,</b> Hematoxylin and eosin images and annotated BaseScope staining for point mutations in PIK3CA (Case C537, top) or KRAS (Case 539, bottom). Scale bars, 5 mm (top) and 1 mm (bottom). Regions circled in green were macrodissected for FUME-TCRseq. <b>B,</b> Bar chart showing the numbers of unique TCR clonotypes detected in the PIK3CA WT and mutant (E545K) subclones of Case C537. <b>C,</b> Bar chart showing the diversity of the TCR repertoire in PIK3CA WT and mutant subclones of Case C537. <b>D,</b> Plot showing the frequencies of the 10 most common clonotypes detected in the PIK3CA E545K mutant subclone and the frequencies at which they appear in the WT subclone. Arrow, TCR sequence CASSYGHPNTEAFF. <b>E,</b> Bar chart showing the numbers of unique TCR clonotypes detected in the KRAS WT and mutant (G12C) subclones of Case C539. <b>F,</b> Bar chart showing the diversity of the TCR repertoire in KRAS WT and mutant subclones of Case C539. <b>G,</b> Plot showing the frequencies of the 10 most common clonotypes detected in the KRAS G12C mutant subclone and the frequencies at which they appear in the WT subclone.</p>