FIGURE 1 from MHC Class II is Induced by IFNγ and Follows Three Distinct Patterns of Expression in Colorectal Cancer Organoids

Abstract

<p>Class II inducibility across cell line and colorectal cancer organoids following IFNγ stimulation. <b>A</b>–<b>C,</b> Flow cytometry assessment of class I and II expression across lines reveal three groups of response to stimulation at 24 hours. Representative plots, with two lines displayed for each group, for noninducible (<10% class II expression; A), weakly inducible (10%–49%; B), and strongly inducible lines (>50%; C). Histogram overlay, right, for class II expression between control (red) and IFNγ (blue). Flow data from all 15 organoids available in <a href="#SMF1" target="_blank">Supplementary Fig. S1</a>. <b>D–F,</b> Class II expression following 24 hours stimulation for all lines, performed in minimum of triplicate, unpaired <i>t</i> test (NS, <i>P</i> ≥ 0.05; *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001). Lines grouped into non/weakly inducible (D), strongly inducible (E), and cell lines (F). <b>G,</b> A total of 72-hour stimulation of weakly and noninducible lines, results in triplicate and statisitcs as above. <b>H,</b> Flow cytometry gating strategy.</p>