FIGURE 1 from Chromatin Accessibility Landscape of Human Triple-negative Breast Cancer Cell Lines Reveals Variation by Patient Donor Ancestry

Abstract

<p>Genome-wide landscape of chromatin accessibility in TNBC cell lines derived from EA and AA women. <b>A,</b> Fragment length distribution plot for TNBC cell lines. Peak < 150 bp indicates a nucleosome-free peak; peak between 150 and 300 bp indicates a mononucleosome peak. FRiP scores greater than 0.3 indicate high quality of the data. Technical replicates (<i>n</i> = 2) were averaged for each cell line. <b>B,</b> Summary of peak distribution across all samples. <b>C,</b> Number of peaks by TNBC cell line. All samples had high peak numbers (∼75,000) covering promoters and intergenic regions. <b>D,</b> Principal component analysis showing separation of the chromosome accessibility landscape by ancestry for the nine TNBC cell lines. Technical replicates (<i>n</i> = 2) were averaged for each line. <b>E,</b> Chromosome map showing the distribution of DARs between EA and AA TNBC cells (FC > 2, FDR < 0.01).</p>