Abstract
Supplemental Figures from Pyataeva et al. 2016. DNA barcodes unite two problematic taxa: the meiobenthic Boreohydra simplex is a life-cycle stage of Plotocnide borealis (Hydrozoa: Aplanulata). Zootaxa. Figure S1. Neighbor joining topology for 16S sequences derived from samples of Plotocnide borealis (n=4) and Boreohydra simplex (n=4), along with all (n=1948) publicly available 16S sequences from exemplars of Hydroidolina in Genbank. Protohydra and Plotocnide/Boreohyra samples are found on page 6 of 27 and highlighted in yellow. The topology shows these taxa diverging near the base of a clade containing all sampled members of Aplanulata. Figure S2. Neighbor joining topology for COI sequences derived from samples of Plotocnide borealis (n=4) and Boreohydra simplex (n=5), along with all (n=1614) publicly available COI sequences from exemplars of Hydroidolina in Genbank. Protohydra samples are found on page 3 of 32, and highlighted in yellow, branching among exemplars of Aplanulata, which do not form a clade in the NJ topology. Plotocnide/Boreohyra samples are highlighted in yellow and found united with COI sequences derived from another putative member of Aplanulata, Margelopsis on page 26 of 32. Figure S3. Maximum likelihood topology for 18S sequences derived from samples of Plotocnide borealis (n=2) and Boreohydra simplex (n=2), along with all (n=519) publicly available 18S sequences from exemplars of Hydroidolina in Genbank. Protohydra and Plotocnide/Boreohyra samples are found on page 10 of 18, branching near and at, respectively, the clade including all exemplars of Aplanulata. Figure S4. Consensus of 420 bootstrap maximum likelihood analyses of 18S sequences derived from samples of Plotocnide borealis (n=2) and Boreohydra simplex (n=2), along with all (n=519) publicly available 18S sequences from exemplars of Hydroidolina in Genbank. Protohydra and Plotocnide/Boreohyra samples are found on pages 8 and 9 of 18, branching near and at, respectively, the clade including all exemplars of Aplanulata.
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