Fight outcome influences male mating success in Drosophila prolongata

Abstract

The outcomes of preceding fights can influence the probability of winning a subsequent fight, known as the winner/loser effect. However, we know relatively little about how the experience of a preceding fight influences subsequent mating success. Here, we investigated the influence of preceding fight outcomes on subsequent mating behavior in a fruit fly Drosophila prolongata. Subordinate males mated less in two-choice mating assays, showing that the fight outcome predicts male mating success in this species. This tendency remained in a no-choice mating assay where direct interaction between the dominant and subordinate males was eliminated, suggesting that the mating disadvantage of the subordinate males was dependent on the experience of the previous fight rather than the direct interference by the dominant male. When a no-choice mating assay was performed before the fight, the prospective subordinate males mated at the same rate as the dominant males, confirming that the intrinsic male qualities in fighting and mating performances were independent of each other in our experiments. These results indicated that the experience-dependent changes in the subordinate males led to the reduced mating success.