Abstract
Among the putative mechanisms, by which extremely low frequency magnetic field (ELF-MF) modify calcium metabolism is that of affecting Ca2+ fluxes across cell membrane or internal Ca2+ stores. To test this hypothesis, whether ELF MF can modulate Ca2+ fluxes of isolated skeletal muscle sarcoplasmic reticulum vesicles (SR) was investigated in the present study. The Ca2+ transport and Ca2+-Mg2+-ATPase activity were observed by means of dynamic Ca2+ dye spectrum, the function of ryanodine receptor (RyR1) was monitored by [3H]-ryanodine binding assay. The membrane fluidity of SR was measured by fluorescence polarization. 50 Hz 0.4 mT MF exposure for 30 min led to a significant decrease in the Ca2+ uptake initial rate and Ca2+-Mg2+-ATPase activity compared to sham exposed SR. These results strongly suggest that prevention of SR Ca2+ uptake by ELF MF exposure was due to the inhibition of Ca2+-Mg2+- ATPase activity, and the increase of SR Ca2+ release was due to the activation of RyR1.
Highlights
With increasing use of electric appliances, high voltage power transmission and magnetic resonance imaging, it has resulted in the increased exposure to a complex mix of artificially elevated extremely low frequency (ELF) magnetic field (MF)
These results strongly suggest that prevention of sarcoplasmic reticulum vesicles (SR) Ca2+ uptake by ELF MF exposure was due to the inhibition of Ca2+-Mg2+ATPase activity, and the increase of SR Ca2+ release was due to the activation of RyR1
Cellular studies have demonstrated that ELF MF can influence processes, such as DNA, RNA or protein synthesis in various cell types [6,7]
Summary
With increasing use of electric appliances, high voltage power transmission and magnetic resonance imaging, it has resulted in the increased exposure to a complex mix of artificially elevated extremely low frequency (ELF) magnetic field (MF). ELF MF have been reported to affect several basic cellular processes, such as cell proliferation [4], apoptosis [5], DNA and RNA synthesis [4,6], and gene transcription [7]. Since all these effects can be related more or less directly to the signal transduction pathways, Ca2+ as an intracellular critical second message has been of focus to scientists for many years [8,9,10,11,12]. Many studies of the effects of ELF MF on cytosolic [Ca2+]c have been completed, the results apparent lack of consistency
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