Abstract
A capillary electrophoretic method for determination of five water soluble vitamins B along with baclofen as an internal standard has been developed and assessed in context of precision, accuracy, sensitivity, freedom from interference, linearity, detection and quantification limits. On-line preconcentration technique, namely field-amplified sample stacking (FASS)–sweeping, has been employed in respect to obtain more sensitive analysis. Separation conditions received after optimization procedure were as following background electrolyte (BGE), 10mM NaH2PO4, 80mM SDS, (pH 7.25); sample matrix (SM), 10mM NaH2PO4 (pH 4.60); uncoated fused silica capillary (50μm i.d.×67cm length); UV spectrophotometric detection at 200nm; injection times: 10s and 30s at 3.45kPa; applied voltage 22kV; temperature 22°C. Validation parameters, namely precision, accuracy and linearity, were considered as satisfactory. Under the optimized conditions, it has been also successfully applied for vitamins B determination in bacterial growth medium and commercially available Ilex paraguariensis leaves.
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