Abstract
IntroductionThe best approach to preventing the importation of coronavirus disease 2019 (COVID-19) is enhancing the detection capacity at customs. The rapid detection is of utmost importance and therefore highly demanded.MethodsWe conducted a field validation study of a duplex real-time reverse transcription recombinase-aided amplification (RT-RAA) assay in Zhoushan and Hangzhou customs, in Zhejiang Province, China. The reverse transcriptase polymerase chain reaction (RT-PCR) assay kit routinely used at customs was used in parallel, and the duration the two methods took to complete a specific number of samples was compared.ResultsAmong 506 samples collected, RT-RAA results were consistent with the RT-PCR results. The sensitivity and specificity were 100%, the total coincidence rate was 100%, and the Kappa value was 1 (P<0.05) for both methods. The RT-RAA kit took a significantly shorter time in testing the 20–200 samples than the RT-PCR kit. DiscussionThe RT-RAA detection method is more efficient and suitable for use at customs than RT-PCR assay to realize rapid customs clearance of 200 or fewer samples.
Highlights
The best approach to preventing the importation of coronavirus disease 2019 (COVID-19) is enhancing the detection capacity at customs
Our findings demonstrate that the reverse transcription recombinase aided amplification (RT-RAA) assay has comparable sensitivity and specificity to the commercial reverse transcriptase polymerase chain reaction (RT-PCR) kits and exhibits the distinctive advantages of simplicity and rapidity regarding operation and turnaround time
SARS-CoV-2 RNA was detected from the clinical samples using the COVID-19 RT-RAA kit, according to the methods described in our previous report [10,11]
Summary
The best approach to preventing the importation of coronavirus disease 2019 (COVID-19) is enhancing the detection capacity at customs. The best approach to preventing the importation of COVID-19 from other countries is enhancing the detection capacity at customs where the detection accuracy and rapidity are highly demanded [4]. Reverse transcriptase polymerase chain reaction (RTPCR) is a commonly used technique at customs for diagnosing COVID-19 [5] This method is time-consuming and requires specialized equipment and skilled personnel [6]. To evaluate the specificity and sensitivity of the RT-RAA kit, the PCR assay kit routinely used at customs was used in parallel, and the duration the two methods took to complete a specific number of samples was compared. This study provides reference data for establishing a rapid on-site detection of SARS-CoV-2 RNA at customs
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