Field study on evaluation of the efficacy and usability of two disinfectants for drinking water treatment at small cattle breeders and dairy cattle farms.

Abstract

The hygienic quality of drinking water for cattle originated from different sources together with the efficacy and usability of two types of disinfectants against waterborne pathogens were assessed for small cattle breeders and dairy cattle farms. A total of 120 drinking water samples were collected from water troughs representing three different water sources commonly used for cattle drinking (tap, underground and surface water; n = 65, 25, and 30, respectively). Collected samples were cultured for isolation and identification of pathogenic bacteria using serological techniques and PCR. The bactericidal efficacy of the disinfectants, sodium dichloroisocyanurate (NaDCC) and hydrogen peroxide (H2O2) 50%, at different concentrations were evaluated by the determination of total viable and coliform counts of water prior and postwater treatment. In small cattle breeders, Escherichia coli was the most prevalent bacterial isolates from surface water (56.7%) followed by Staphylococcus aureus (36.7%), Salmonella spp. (26.7%), Streptococcus faecalis (23.3%), Shigella flexneri (16.7%), Proteus spp. (16.7%), and Klebsiella pneumonae (10.0 %) at X(2) = 9, P ≤ 0.01. Prior to the use of disinfectants, the averages of total bacterial and coliform counts were the highest in surface water (3.56 × 10(7), 240.0, and 38.0 CFU/100 ml, respectively). It has been found that hydrogen peroxide 50% at a concentration of 35 mg/l had a lethal effect (100 %) on indicator microorganisms compared with NaDCC at concentration of 2 mg/l. In conclusion, the higher bacterial contaminants in drinking water were found in surface water followed by tap water, particularly for small cattle breeders. Therefore, the usage of more hygienic water troughs with their regular treatment by hydrogen peroxide 50% at concentration of 35 mg/l is highly recommended to control waterborne bacteria and consequently improve and maintain the animal health.