Abstract
A two-stage, field-scale bioreactor system was used to determine the efficacy of bioremediation of creosote- and pentachlorophenol (PCP)-contaminated ground water at the abandoned American Creosote Works (ACW) site in Pensacola, Florida. In separate 15-day runs of the field-scale (454L) system, bioreactor performance in the presence of specially-selected microbial inoculants was compared to that observed using non-specific biomass. In the first run, Bioreactor #1 was amended with nutrients, surfactants and microorganisms (strains CRE 1–13) that were isolated from soil at ACW and selectively cultured for their ability to biodegrade monitored creosote constituents. After 4 days of organism acclimation and degradation of organic contaminants, the batch system was converted to a flow-through regime. Effluent was transferred to Bioreactor #2 where Pseudomonas paucimobilis strain EPA 505 and Pseudomonas sp. strain SR 3 were introduced. A second 15-day bioreactor run was conducted using indigenous bacteria and microorganisms from a waste water treatment facility that was designed to treat effluents containing PAHs and phenolics.
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