Abstract
Abstract We used plastic bags to incubate, transport, and hatch embryos for specific crosses of razorback suckers Xyrauchen texanus during two spawning seasons on the Colorado River (Nevada–Arizona). Fifty to 2,000 razorback sucker eggs were fertilized in plastic food storage bags for 26 matings in 102 trials. Experimental treatments prevented hatching in all bags, but hatching was observed when fresh milt and viable eggs were used (10–99% hatch). Observation of embryo development and treatment effects was facilitated by the transparency and portability of the bags. Water volume and exchange rates were chosen according to estimated metabolic rates, and the addition of oxygen to the bags was evaluated to increase holding times. Plastic bags were inexpensive and useful for gamete storage, controlled propagation, and transport of gametes, embryos, and fry under field conditions. These techniques are suitable for various applications in studies of reproductive biology, and they are especially suited to geneti...
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