Abstract
A new method for maintaining the isolated frog optic tectum superfused in vitro at a low temperature is described. The preparation remains viable for several hours with electrophysiological responses similar to those of the in vivo tectum. This superfusion technique will allow controlled application of putative neurotransmitters and other substances in known concentrations and therefore makes the in vitro frog optic tectum a useful model system for neuropharmacological investigations into central optic pathways.
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