Abstract
Fundacion para el Fomento y Promocion de la Investigacion y Transferencia de Tecnologia Agropecuaria de Costa Rica/[F15-16]/FITTACORI/Costa Rica
Highlights
Sex determination of papaya is important because markets prefer the hermaphrodite fruit shape, which tends to be pear-shaped or elongated
To test the growth and production of hermaphrodite plants derived from molecular markers and micropropagation, two independent field experiments were conducted at the same location and the performance compared with that of plants selected by the conventional sex determination method, by which four plants per hole are thinned to one
Each primer set was optimized to a final concentration of 0.075 μM for P01F-R and P02F-R, Field performance of hermaphrodite papaya plants obtained through molecular selection and micropropagation combined with 0.3 μM for SDP-1 and SDP-2
Summary
Sex determination of papaya is important because markets prefer the hermaphrodite fruit shape, which tends to be pear-shaped or elongated. In Costa Rica, the W11 marker allowed sex determination of 1500 seedlings of the hybrid ‘Pococí’, where the observed female: hermaphrodite plant ratio did not differ from the expected 1:1 segregation (Saalau-Rojas et al 2009). This study compared the methods end-point PCR and qPCR for sex determination of the papaya hybrid ‘Pococí’.
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