Field gradient techniques in NMR spectroscopy

Abstract

In recent years NMR spectroscopy has emerged as a powerful technique for studying the structure and dynamics of biomolecules in solution. The development of shielded pulsed-field gradient coils for high-resolution NMR spectroscopy has led to significant improvements in a large number of experiments which are used to provide such information. Experiments enhanced with pulsed-field gradients have fewer artifacts, suffer far less from problems of solvent suppression and have reduced phase cycles relative to their non-gradient counterparts. The whole array of NMR experiments available for macromolecular structure determination is likely to benefit substantially from the incorporation of gradient pulses.