Abstract
Polerovirus: potato leaf roll virus (PLRV), Potyvirus: potato virus Y (PVY) and Potexvirus: potato virus X (PVX) is more destructive and well distributed throughout the Pakistan. Incidence has been reported to be as high as 90%, 25%, and ≥ 15%, respectively in the potato growing regions. To find out the source of resistance, twenty-nine virus free potato varieties were grown under field conditions with good agricultural practices. The disease severity of PLRV, PVY and PVX was recorded to determine the level of resistance of the potato varieties according to the disease rating scale. Infectivity and biological assay of all twenty-nine varieties were done in green house on potato, Datura stramonium, Nicotiana glutinosa and Physalis floridana. Non-inoculated plants were served as control. Leaf samples from potato varieties were collected for serological detection of PLRV, PVY and PVX by Double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). Out of twenty nine varieties, none of the variety was resistant to PLRV although three varieties; Mirrato, 394021-120 and Orla were moderately resistant. Only FD 48-4 and TPS 9813 showed resistance to PVX and PVY. While FD 3-10, FD 9616 and FD 37-13 were moderately to PVX and PVY. Rest of the varieties was found susceptible to all three viruses.
Highlights
Potato (Solanum tuberosum L.) is the world’s third most important food crop and widely distributed (Bhaskar et al, 2010) due to its high production and good nutritional value
Twenty-nine varieties of virus free potato obtained from Potato Research Institute Sahiwal, Punjab, Pakistan, were evaluated for their resistance against potato leaf roll virus (PLRV), potato virus Y (PVY) and potato virus X (PVX)
Disease severity of PLRV, PVY and PVX infected plants showing symptoms were determined by visual inspection
Summary
Potato (Solanum tuberosum L.) is the world’s third most important food crop and widely distributed (Bhaskar et al, 2010) due to its high production and good nutritional value. Several diseases hamper its production (Ahmed & Bhutta, 1989). Among these most important and extensively widespread are the aphid-transmissible RNA viruses (Robert, 2000; Kogovsek et al, 2010), i.e. Polerovirus: potato leaf roll virus (PLRV), Potyvirus: potato virus Y (PVY) and non aphid-transmissible RNA virus (Adams et al, 2004) Potexvirus: potato virus X (PVX). PLRV is transmitted in a circulative, persistent and non-propagative manner by its most important vector aphid; Myzus persicae Sulz (Salazar, 2003). PVY is transmitted mechanically and by aphid (Myzus persicae) in a non-persistent manner (Jones et al, 2009; Kerlan & Moury, 2009). Most prominent symptom is the ‘leaf-drop streak’ or necrosis along the veins of the underside of leaflets and leaf jas.ccsenet.org
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