Abstract
A BioFET (biological field effect transisor) for detection of C-reactive protein has been characterised using cysteine-tagged protein G capable of site-specific immobilisation of antibody to enhance antigen detection. Drain current of the BioFET with immobilised protein G on the gate surface has been measured in different pH solutions to investigate charge effect of the protein. Antibody–antigen interaction was observed through variation of the drain current of the BioFET. The current differential ratio was linearly related to the concentration of the C-reactive protein in the range 3–20 µg/ml.
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