Field Collection and Short-Term Storage of Walleye Semen

Abstract

Abstract The goal of this research project was to develop approaches and methods for large-scale field collection and short-term storage of semen obtained from walleyes (Stizostedion vitreum). Male walleyes were most effectively collected by trap-netting in shallow water (1–2 m deep). Most fish (78%n) were collected at water temperatures higher than 6°C. The ratio of males to females in the catch was inversely related to water temperature. An efficient concentration of tricaine methanesulfonate (MS-222) for anesthesia prior to stripping was established at 99 mg!L; time to immobilization averaged 211 s. Average amounts of semen produced per fish from first, second, and third daily strippings were 3.1, 1.3, and 0.5 mL, respectively. Semen was preserved in extender (1 part fresh semen : 2 parts extender) and stored in plastic sandwich containers (11.4 × 10.8 cm). Each container held 24 mL of preserved semen. Eggs were fertilized with 4.0 mL of extended semen per liter of eggs. Over a 5-year period, hatching ...