Field and laboratory investigations on factors affecting the diel variation of arsenic in Huangshui Creek from Shimen Realgar Mine, China: implications for arsenic transport in an alkali stream.

Abstract

The release of arsenic and related species from mining activities has been investigated widely at both seasonal and diel scales, contributing to the understanding of arsenic cycles, its ultimate fate, and enabling accurate estimates of arsenic flux in specific areas. To enrich the research in this area, a case study was undertaken in Huangshui Creek, Hunan province, China. Here, arsenic is present in the sediment at the Creek entrance to a reservoir and in the widely developed alkali realgar(α-As4S4)-calcite(CaCO3)-dolomite[CaMg(CO3)2] strata (pH 7-11). Water from different levels in the Huangshui Creek, the Creek/reservoir entrance, and the downstream reservoir together with corresponding sediments were collected and analyzed. The local algae were separated and cultured. A diel variation of arsenic (688 ug/L in AM 3:50-1152 ug/L in PM 19:50) was observed in the Creek. The largest difference in arsenic concentration between the upper and lower water body was at the mixed creek/reservoir site (364 ug/L). Laboratory experiments showed that arsenic release from Creek sediment and pristine realgar was 1.3-2.7 times and 2.0-2.3 times at 25 and 37°C, respectively, than low-temperature samples (8°C) over 24h. However, temperature variation is not the only factor controlling arsenic release from Huangshui Creek. Batch experiments show that both sediment and pristine realgar can release arsenic(III). In addition, the presence of bicarbonate promotes arsenic(V) release by 15.2-24.3 times for the sediment and by 1.7-3.4 times for pristine realgar compared to the control, though it restrains arsenic(III) release. High levels of algae have a complex effect on arsenic release; it increases arsenic(V) release by accelerating dissolution of realgar but decreases arsenic(III) release through adsorption. The field observations-variation of bicarbonate (67mg/L in day and 201mg/L in night) and chlorophyll-a (0.06-0.87)-support that both dissolved bicarbonate and algae affect arsenic concentration. These factors establish a circadian rhythm in the Creek, which coupled with arsenic release, ultimately affect the fate of arsenic.